Silva Fennica : regular issues : 44(2) : sa442197.htm

Juha Kaitera & Heikki Nuorteva. 2010.

Effects of Melampyrum extracts on the growth of axenic cultures of Cronartium flaccidum and Peridermium pini

Silva Fennica 44(2): 197–202

For 3–6 months, mycelial colonies cultured from 5 isolates of each of two pine stem rusts (Cronartium flaccidum and Peridermium pini) were grown on nutrient-rich agar supplemented with Melampyrum extracts. Non-autoclaved extracts of M. pratense significantly reduced the growth of P. pini. The growth of C. flaccidum isolates was slightly stimulated after the second month of incubation but after that was inhibited during incubation months 4–6. We observed considerable variation in colony growth, a significant component of which was explained by incubation time, isolate, growth medium and their interaction. Rust species (C. flaccidum or P. pini) was not an important factor in growth variation. While sterilized extracts of M. pratense, M. sylvaticum and M. nemorosum did not significantly affect growth, colonies of C. flaccidum were slightly stimulated, whereas colonies of P. pini were slightly inhibited. Generally, isolates of P. pini grew better and showed a slower rate of degeneration than C. flaccidum on all media.

Keywords
alternate host, anti-fungal phytochemicals, fungal growth regulation, pine stem rust, Scots pine

Addresses
Kaitera, Finnish Forest Research Institute, Northern Finland Regional Unit, FI-91500 Muhos, Finland; Nuorteva, Finnish Forest Research Institute, Southern Finland Regional Unit, FI-01301 Vantaa, Finland. E-mail juha.kaitera@metla.fi

Received 12 October 2009 Revised 19 February 2010 Accepted 22 February 2010
ISSN 0037-5330

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