Silva Fennica : quarterly issues : 30(2–3) : sa3023123.htm

Virpi Palomäki, Kaisa Laitinen, Toini Holopainen & Seppo Kellomäki. 1996.

First-year results on the effects of elevated atmospheric CO2 and O3 concentrations on needle ultrastructure and gas exchange responses of Scots pine saplings

Silva Fennica 30(2–3): 123–134

The effects of realistically elevated O3 and CO2 concentrations on the needle ultrastructure and photosynthesis of ca. 20 year-old Scots pine (Pinus sylvestris L.) saplings were studied during one growth period in open-top field chambers situated on a natural pine heath at Mekrijärvi, in eastern Finland. The experiment included six different treatments: chamberless control, filtered air, ambient air and elevated O3, CO2 and O3 + CO2. Significant increases in the size of chloroplast and starch grains were recorded in the current-year needles of the saplings exposed to elevated CO2. These responses were especially clear in the saplings exposed to elevated O3 + CO2 concentrations. These treatments also delayed the winter hardening process in cells. In the shoots treated with O3, CO2 and combined O3 + CO2 the Pmax was decreased on average by 50% (ambient CO2) and 40% (700 ppm CO2). Photosynthetic efficiency was decreased by 60% in all the treated shoots measured under ambient conditions and by 30% in the CO2 and O3 + CO2 treated shoots under 700 ppm. The effect of all the treatments on photosynthesis was depressive which was probably related to evident accumulation of starch in the chloroplasts of the pines treated with CO2 and combined O3 + CO2. But in O3 treated pines, which did not accumulate starch in comparison to pines subjected to ambient air conditions, some injuries may be already present in the photosynthetic machinery.

CO2, O3, Scots pine, needle ultrastructure, gas exchange, climate change

Palomäki and Holopainen, University of Kuopio, Department of Ecology and Environmental Science, P.O.Box 1627, FIN-70211 Kuopio, Finland. Laitinen and Kellomäki, University of Joensuu, Faculty of Forestry, P.O. Box 111, FIN-80101 Joensuu, Finland. Fax +358 17 163 230, E-mail

Received 22 September 1995 Accepted 18 June 1996
ISSN 0037-5330