Forest list archive: msg00060

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ECTOMYCORRHIZAE STAIN ?



Dear Netters,

I am trying to quantify (count) short mycorrhizal and short 
non-mycorrhizal roots on the root samples of loblolly and slash pine 
seedlings which I grew in a nursery bed.  Half of the seedlings were 
inoculated with *Pisolithus tinctorius* at planting.  The major 
difficulty which I encounter looking at the root samples under a 
disecting microscope (magnification 10x to 140x) is that I don't 
know if monopodial short roots which are club shaped/swollen toward the 
root tip are or are not mycorrhizal.

Does any one know a fast method of identifying ectomycorrhizal roots by 
STAINING or any other method?  Would larger magnification and 
cross-sectioning help to properly classify the roots in question?

Please, respond to my personnal address: jn1@Ra.MsState.Edu, I will post 
the summary if there will be enough interest expressed.

Jarek Nowak
Graduate Research Assistant
Department of Forestry
Mississippi State University


   




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