germinating lodgepole pine seeds

• To: forest@nic.funet.fi
• Subject: germinating lodgepole pine seeds
• From: devans@nancy.inra.fr
• Date: 22 Dec 92 8:32
• Content-Length: 803

I need to germinate small numbers of lodgepole pine (_Pinus contorta_)
seeds in axenic conditions. I have tried surface sterilisation with
Hydrogen Peroxide (30% for 20 minutes) followed by plating onto 1%glucose
agar plates. These were then kept at 25 C in darkness. However germination
rate was very low (2 - 5 %).
Does any one know of a better method ? or of some references which may give
details.
Thanks for any help,
Doug


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* Doug Evans					DEVANS@NANCY.INRA.FR       *
* Laboratoire de microbiologie forestiere,	Tel 83 39 40 41            *
* INRA-Nancy,					Fax 83 39 40 69            *
* 54280 Champenoux, 							   *
* France.								   *
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